Zeiss Light Sheet 7
Light Sheet Fluorescence Microscopy (LSFM) is an extremely powerful alternative to established fluorescence imaging techniques, especially for 3-D imaging within whole live organisms and large tissue explants. Ideal for fast and gentle imaging of whole living model organisms, tissues, and cells as they develop – over extended periods of time capable of imaging large optically cleared specimens in toto – with subcellular resolution. By selectively illuminating the observed optical section with a thin sheet of light, photo bleaching is reduced to a minimum, making light sheet microscopy ideal for nondestructive imaging of fragile samples over extended periods of time. Equipped with an incubation system with temperature and CO2 control for long-term in vivo experiments.
Available Objectives:
| Magnification | Objestive Type | Description | NA | Immersion | WD (mm) |
| 2.5 | Fluar | 2.5x | 0.12 | Clearing | 8.7 |
| 5 | EC Plan-Neofluar | 5x | 0.16 foc | Water/Clearing | 18.5 |
| 5 | EC Plan-Neofluar | 5x | 0.16 | Clearing | 18.5 |
| 10 | W Plan-Apochromat | 10x | 0.50 | Water | 3.7 |
| 20 | W Plan-Apochromat | 20x | 1.00 | Water | 2.4 |
| 20 | Clr Plan-Neofluar | 20x | 1.00 | Clearing | 6.4 |
Lasers lines wavelengths (nm):
405, 445, 488, 514, 561, 638
Camera:
Two liquid cooled sCMOS cameras, pco.EDGE
Filter Sets:
| Fluorochromes | Mirror | Filter 1 (nm) | Filter 2 (nm) |
| DAPI/GFP | SBS LP 490 | BP 420-470 (DAPI) | BP 505-545 (GFP) |
| DAPI/RFP | SBS LP 510 | BP 420-470 (DAPI) | BP 575-615 (RFP) |
| GFP/mCherry | SBS LP 560 | BP 505-545 (GFP) | LP 585 (mCherry) |
| GFP/ Draq5(toto3) | SBS LP 560 | BP 505-545 (GFP) | LP 660 (Draq5(toto3)) |
| RFP/ Draq5(toto3) | SBS LP 640 | BP 575-615 (RFP) | LP 660 (Draq5(toto3)) |
Available Capillary Tubes
Red = 0.68 mm
Black = 1.00 mm
Green = 1.50 mm
Blue = 2.15 mm